检测认证人脉交流通讯录
Chroma 激光专用滤光片 FISH滤光片 TIRF滤光片 Raman滤光片
- 主要规格:
- FISH滤光片 TIRF滤光片 Raman滤光片
- 用 途:
- 用于显微镜,观察FISH TIRF Raman现象
- Chroma激光专用滤光片 FISH滤光片 TIRF滤光片 Raman滤光片是常见的集中滤光片类型,属于对光学品质要求较高的滤光片。
激光专用滤光片用于激光作为光源的显微镜,例如激光扫描共聚焦荧光显微镜(LSCM),全内反射滤光片(TIRF)用于全内反射荧光显微镜(TIRFM),拉曼滤光片用于拉曼成像(Raman),全内反射荧光片用于荧光原位杂交分析(FISH)。
一、荧光显微镜 LSCM显微镜 TIRFM显微镜 FISH荧光原为杂交法 Raman光谱分析
荧光显微镜(Fluorescence microscope) : 荧光显微镜是以紫外线为光源, 用以照射被检物体, 使之发出荧光, 然后在显微镜下观察物体的形状及其所在位置。荧光显微镜用于研究细胞内物质的吸收、运输、化学物质的分布及定位等。 细胞中有些物质,如叶绿素等,受紫外线照射后可发荧光;另有一些物质本身虽不能发荧光,但如果用荧光染料或荧光抗体染色后,经紫外线照射亦可发荧光,荧光显微镜就是对这类物质进行定性和定量研究的工具之一。
激光扫描共聚焦荧光显微镜(laser scanning confocal microscopy,LSCM)
测针孔和光源针孔始终聚焦于同一点,使聚焦平面以外被激发的荧光不能进入检测针孔。
激光共聚焦的工作原理简单表达就是它采用激光为光源,在传统荧光显微镜成像的基础上,附加了激光扫描装置和共轭聚焦装置,通过计算机控制来进行数字化图像采集和处理的系统。
TIRFM 全内反射荧光显微镜
全内角反射荧光显微镜(total internal reflection fluorescence microscope,TIRFM),利用光线全反射后在介质另一面产生衰逝波的特性,激发荧光分子以观察荧光标定样品的极薄区域,观测的动态范围通常在200 nm以下。因为激发光呈指数衰减的特性,只有极靠近全反射面的样本区域会产生荧光反射,大大降低了背景光噪声干扰观测标的,能帮助研究者获得高质量的成像质量和可靠的观测数据。故此项技术广泛应用于细胞表面物质的动态观察。
二、激光专用滤光片 FISH滤光片 FITR滤光片 Raman滤光片 NF滤光片的定义
ND滤光片
中性灰度镜(neutral density filter)又叫中灰密度镜,简称ND镜,其作用是过滤光线。这种滤光作用是非选择性的,也就是说,ND镜对各种不同波长的光线的减少能力是同等的、均匀的,只起到减 弱光线的作用,而对原物体的颜色不会产生任何影响,因此可以真实再现景物的反差。
FISH滤光片
荧光原位杂交技术(Fluorescence in situ hybridization, FISH)是根据已知微生物不同分类级别上种群特异的DNA序列,以利用荧光标记的特异寡聚核苷酸片段作为探针,与环境基因组中DNA分子杂交,检测该特异微生物种群的存在与丰度。
NF滤光片
陷波滤光片,通常也被称作带阻或者带抑制滤光片。它可以透过绝大多数波长,但是将特定波长范围内(阻带)的光衰减到非常低的水平。拉曼光谱用。
Chroma激光专用滤光片 FISH滤光片 FITR滤光片 Raman滤光片的型号参数
荧光滤光片型号 滤光片类型 滤光片说明 注释(燃料,长通或短通)
Blue-27M AS 27mm diameter, mounted in 1.25" threaded ring
Ha/8-27M AS 27mm diameter, mounted in 1.25" threaded ring
Lum-27M AS 27mm diameter, mounted in 1.25" threaded ring
Red-27M AS 27mm diameter, mounted in 1.25" threaded ring
Green-27M AS 27mm diameter, mounted in 1.25" threaded ring
Ha/8-31 AS 31mm diameter, unmounted
Red-31 AS 31mm diameter, unmounted
Blue-31 AS 31mm diameter, unmounted
Green-31 AS 31mm diameter, unmounted
Lum-31 AS 31mm diameter, unmounted
Lum-36 AS 36mm diameter, unmounted
Green-36 AS 36mm diameter, unmounted
Blue-36 AS 36mm diameter, unmounted
Red-36 AS 36mm diameter, unmounted
Ha/8-36 AS 36mm diameter, unmounted
Red-50 AS 50mm diameter, unmounted
Lum-50 AS 50mm diameter, unmounted
Ha/8-50 AS 50mm diameter, unmounted
Green-50 AS 50mm diameter, unmounted
Green-50S AS 50mm x 50mm, square
Blue-50S AS 50mm x 50mm, square
Ha/8-50S AS 50mm x 50mm, square
Lum-50S AS 50mm x 50mm, square
Red-50S AS 50mm x 50mm, square
Ha/8-65S AS 65mm x 65mm, square
Red-65S AS 65mm x 65mm, square
Lum-65S AS 65mm x 65mm, square
Blue-65S AS 65mm x 65mm, square
Green-65S AS 65mm x 65mm, square
Green-27M AS 27mm diameter, mounted in 1.25" threaded ring
Red-27M AS 27mm diameter, mounted in 1.25" threaded ring
Lum-27M AS 27mm diameter, mounted in 1.25" threaded ring
Blue-27M AS 27mm diameter, mounted in 1.25" threaded ring
Lum-31 AS 31mm diameter, unmounted
Green-31 AS 31mm diameter, unmounted
Blue-31 AS 31mm diameter, unmounted
Red-31 AS 31mm diameter, unmounted
Red-36 AS 36mm diameter, unmounted
Blue-36 AS 36mm diameter, unmounted
Lum-36 AS 36mm diameter, unmounted
Green-36 AS 36mm diameter, unmounted
Green-50 AS 50mm diameter, unmounted
Lum-50 AS 50mm diameter, unmounted
Red-50 AS 50mm diameter, unmounted
Red-50S AS 50mm x 50mm, square
Lum-50S AS 50mm x 50mm, square
Green-50S AS 50mm x 50mm, square
Blue-50S AS 50mm x 50mm, square
Green-65S AS 65mm x 65mm, square
Blue-65S AS 65mm x 65mm, square
Red-65S AS 65mm x 65mm, square
Lum-65S AS 65mm x 65mm, square
HQ493/16x EX Spectrum Green™
HQ527/30m EM Spectrum Green™
Q507lp BS Spectrum Green™
HQ548/20x EX Spectrum Orange™
HQ580/14m EM Spectrum Orange™
Q565lp-AR BS Spectrum Orange™
400dclp-AR BS DAPI
D470/40m-AR EM DAPI
AT350/50x EX DAPI
HQ522/40m EM FITC
Q497lp BS FITC
HQ471/39x EX FITC
Q557lp BS Cy3™ v1
HQ567/15m EM Cy3™ v1
HQ546/11x EX Cy3™ v1
HQ565/18m EM Cy3™ v2
HQ530/30x EX Cy3™ v2
Q553lp BS Cy3™ v2
Q593lp BS Cy3.5™ v1
HQ581/10x EX Cy3.5™ v1
HQ617/40m EM Cy3.5™ v1
HQ617/40m EM Cy3.5™ v2
HQ580/13x EX Cy3.5™ v2
Q593lp BS Cy3.5™ v2
Q647lp BS Cy5™ v1
HQ622/36x EX Cy5™ v1
HQ667/30m EM Cy5™ v1
HQ667/30m EM Cy5™ v2
HQ630/20x EX Cy5™ v2
Q649lp BS Cy5™ v2
Q697lp BS Cy5.5™
HQ682/12x EX Cy5.5™
HQ721/42m EM Cy5.5™
HQ740/35x EX Cy7™
HQ795/50m EM Cy7™
Q765lp BS Cy7™
Q615lp BS Texas Red®
HQ631/23m EM Texas Red®
HQ602/13x EX Texas Red®
HQ782/20x EX Laser-Pro
HQ817/25m EM Laser-Pro
Q800lp BS Laser-Pro
ET460/50m EM ET - 405nm Laser Bandpass Set for TIRF applications
ZET405/20x EX ET - 405nm Laser Bandpass Set for TIRF applications
ZT405rdc BS ET - 405nm Laser Bandpass Set for TIRF applications
ET425lp EM ET - 405nm Laser Bandpass Set for TIRF applications
ET460lp EM ET - 440nm Laser Bandpass Set for TIRF applications
ZET436/20x EX ET - 440nm Laser Bandpass Set for TIRF applications
ZT442rdc BS ET - 440nm Laser Bandpass Set for TIRF applications
ET480/40m EM ET - 440nm Laser Bandpass Set for TIRF applications
ZT442rdc BS ET - 440nm Laser Bandpass Set for TIRF applications
ET500/40m EM ET - 440nm Laser Bandpass Set for TIRF applications
ZET436/20x EX ET - 440nm Laser Bandpass Set for TIRF applications
ET460lp EM ET - 440nm Laser Bandpass Set for TIRF applications
ZT488rdc BS ET - 488nm Laser Bandpass Set for TIRF applications
ET500lp EM ET - 488nm Laser Bandpass Set for TIRF applications
ET525/50m EM ET - 488nm Laser Bandpass Set for TIRF applications
ZET488/10x EX ET - 488nm Laser Bandpass Set for TIRF applications
ZT514rdc BS ET - 514nm Laser Bandpass Set for TIRF applications
ZET514/10x EX ET - 514nm Laser Bandpass Set for TIRF applications
ET545/40m EM ET - 514nm Laser Bandpass Set for TIRF applications
ET525lp EM ET - 514nm Laser Bandpass Set for TIRF applications
ET542lp EM ET - 532nm Laser Bandpass Set for TIRF applications
ET575/50m EM ET - 532nm Laser Bandpass Set for TIRF applications
ZT532rdc BS ET - 532nm Laser Bandpass Set for TIRF applications
ZET532/10x EX ET - 532nm Laser Bandpass Set for TIRF applications
ZET532/10x EX ET - 532nm Laser Bandpass Set for TIRF applications
ZT532rdc BS ET - 532nm Laser Bandpass Set for TIRF applications
ET590/50m EM ET - 532nm Laser Bandpass Set for TIRF applications
ET542lp EM ET - 532nm Laser Bandpass Set for TIRF applications
ZET561/10x EX ET - 561nm Laser Bandpass Set for TIRF applications
ZT561rdc BS ET - 561nm Laser Bandpass Set for TIRF applications
ET600/50m EM ET - 561nm Laser Bandpass Set for TIRF applications
ET575lp EM ET - 561nm Laser Bandpass Set for TIRF applications
ET575lp EM ET - 561nm Laser Bandpass Set for TIRF applications
ZT561rdc BS ET - 561nm Laser Bandpass Set for TIRF applications
ZET561/10x EX ET - 561nm Laser Bandpass Set for TIRF applications
ET630/75m EM ET - 561nm Laser Bandpass Set for TIRF applications
ZT594rdc BS ET - 594nm Laser Bandpass Set for TIRF applications
ET645/75m EM ET - 594nm Laser Bandpass Set for TIRF applications
ZET594/10x EX ET - 594nm Laser Bandpass Set for TIRF applications
ET610lp EM ET - 594nm Laser Bandpass Set for TIRF applications
ET655lp-TRF EM ET - 633-640nm Laser Longpass Set for TIRF applications
ZET635/20x EX ET - 633-640nm Laser Longpass Set for TIRF applications
ZT640rdc BS ET - 633-640nm Laser Longpass Set for TIRF applications
ZET642/20x EX ET - 640-647nm Laser Longpass Set for TIRF applications
ZT647rdc BS ET - 640-647nm Laser Longpass Set for TIRF applications
ET665lp EM ET - 640-647nm Laser Longpass Set for TIRF applications
ET705/72m EM ET - 640-647nm Laser Longpass Set for TIRF applications
ZT405/488rpc BS ET – 405/488nm Laser Dual Band Set for TIRF applications
ZET405/488x EX ET – 405/488nm Laser Dual Band Set for TIRF applications
ZET405/488m-TRF EM ET – 405/488nm Laser Dual Band Set for TIRF applications
ZET405/488x EX ET – 405/488nm Laser Dual Band Set for TIRF applications
ZET405/488m EM ET – 405/488nm Laser Dual Band Set for TIRF applications
ZT405/488rpc BS ET – 405/488nm Laser Dual Band Set for TIRF applications
ET450/50m EM ET – 405/488nm Laser Dual Band Set for TIRF applications
ET525/50m EM ET – 405/488nm Laser Dual Band Set for TIRF applications
ZET442/514m-TRF EM ET – 442/514nm Laser Dual Band Set for TIRF applications
ZT442/514rpc BS ET – 442/514nm Laser Dual Band Set for TIRF applications
ZET442/514x EX ET – 442/514nm Laser Dual Band Set for TIRF applications
ZET442/514x EX ET – 442/514nm Laser Dual Band Set for TIRF applications
ZT442/514rpc BS ET – 442/514nm Laser Dual Band Set for TIRF applications
ZET442/514m EM ET – 442/514nm Laser Dual Band Set for TIRF applications
ET545/40m EM ET – 442/514nm Laser Dual Band Set for TIRF applications
ET480/40m EM ET – 442/514nm Laser Dual Band Set for TIRF applications
ZET442/532x EX ET – 442/532nm Laser Dual Band Set for TIRF applications
ZT442/532rpc BS ET – 442/532nm Laser Dual Band Set for TIRF applications
ZET442/532m-TRF EM ET – 442/532nm Laser Dual Band Set for TIRF applications
ET585/65m EM ET – 442/532nm Laser Dual Band Set for TIRF applications
ZT442/532rpc BS ET – 442/532nm Laser Dual Band Set for TIRF applications
ZET442/532x EX ET – 442/532nm Laser Dual Band Set for TIRF applications
ZET442/532m EM ET – 442/532nm Laser Dual Band Set for TIRF applications
ET500/40m EM ET – 442/532nm Laser Dual Band Set for TIRF applications
ZET488/561x EX ET – 488/561nm Laser Dual Band Set for TIRF applications
ZET488/561m-TRF EM ET – 488/561nm Laser Dual Band Set for TIRF applications
ZT488/561rpc BS ET – 488/561nm Laser Dual Band Set for TIRF applications
ZT488/561rpc BS ET – 488/561nm Laser Dual Band Set for TIRF applications
ET610/75m EM ET – 488/561nm Laser Dual Band Set for TIRF applications
ZET488/561m EM ET – 488/561nm Laser Dual Band Set for TIRF applications
ZET488/561x EX ET – 488/561nm Laser Dual Band Set for TIRF applications
ET525/50m EM ET – 488/561nm Laser Dual Band Set for TIRF applications
ZET488/594x EX ET – 488/594nm Laser Dual Band Set for TIRF applications
ZT488/594rpc BS ET – 488/594nm Laser Dual Band Set for TIRF applications
ZET488/594m-TRF EM ET – 488/594nm Laser Dual Band Set for TIRF applications
ZT488/594rpc BS ET – 488/594nm Laser Dual Band Set for TIRF applications
ZET488/594m EM ET – 488/594nm Laser Dual Band Set for TIRF applications
ZET488/594x EX ET – 488/594nm Laser Dual Band Set for TIRF applications
ET645/75m EM ET – 488/594nm Laser Dual Band Set for TIRF applications
ET525/50m EM ET – 488/594nm Laser Dual Band Set for TIRF applications
ZET488/640x EX ET – 488/640nm Laser Dual Band Set for TIRF applications
ZT488/640rpc BS ET – 488/640nm Laser Dual Band Set for TIRF applications
ZET488/640m-TRF EM ET – 488/640nm Laser Dual Band Set for TIRF applications
ZT488/640rpc BS ET – 488/640nm Laser Dual Band Set for TIRF applications
ZET488/640m EM ET – 488/640nm Laser Dual Band Set for TIRF applications
ZET488/640x EX ET – 488/640nm Laser Dual Band Set for TIRF applications
ET655lp EM ET – 488/640nm Laser Dual Band Set for TIRF applications
ET525/50m EM ET – 488/640nm Laser Dual Band Set for TIRF applications
ZET532/640x EX ET – 532/640nm Laser Dual Band Set for TIRF applications
ZT532/640rpc BS ET – 532/640nm Laser Dual Band Set for TIRF applications
ZET532/640m-TRF EM ET – 532/640nm Laser Dual Band Set for TIRF applications
ET585/65m EM ET – 532/640nm Laser Dual Band Set for TIRF applications
ZT532/640rpc BS ET – 532/640nm Laser Dual Band Set for TIRF applications
ZET532/640x EX ET – 532/640nm Laser Dual Band Set for TIRF applications
ZET532/640m EM ET – 532/640nm Laser Dual Band Set for TIRF applications
ET655lp EM ET – 532/640nm Laser Dual Band Set for TIRF applications
ZET405/488/561x EX ET - PALM Dual Band Emission Set for 488/561nm TIRF applications with 405nm excitation
ZT405/488/561rpc BS ET - PALM Dual Band Emission Set for 488/561nm TIRF applications with 405nm excitation
ET575lp EM ET - PALM Dual Band Emission Set for 488/561nm TIRF applications with 405nm excitation
ZET488/561m EM ET - PALM Dual Band Emission Set for 488/561nm TIRF applications with 405nm excitation
ET525/50m EM ET - PALM Dual Band Emission Set for 488/561nm TIRF applications with 405nm excitation
ZT405/488/561rpc BS ET - 405/488/561nm Laser Triple Band Set for TIRF applications
ZET405/488/561x EX ET - 405/488/561nm Laser Triple Band Set for TIRF applications
ZET405/488/561m-TRF EM ET - 405/488/561nm Laser Triple Band Set for TIRF applications
ZET405/488/561m EM ET - 405/488/561nm Laser Triple Band Set for TIRF applications
ZET405/488/561x EX ET - 405/488/561nm Laser Triple Band Set for TIRF applications
ZT405/488/561rpc BS ET - 405/488/561nm Laser Triple Band Set for TIRF applications
ET575lp EM ET - 405/488/561nm Laser Triple Band Set for TIRF applications
ET450/50m EM ET - 405/488/561nm Laser Triple Band Set for TIRF applications
ET525/50m EM ET - 405/488/561nm Laser Triple Band Set for TIRF applications
ZET405/488/594x EX ET - 405/488/594nm Laser Triple Band Set for TIRF applications
ZT405/488/594rpc BS ET - 405/488/594nm Laser Triple Band Set for TIRF applications
ZET405/488/594m-TRF EM ET - 405/488/594nm Laser Triple Band Set for TIRF applications
ZT405/488/594rpc BS ET - 405/488/594nm Laser Triple Band Set for TIRF applications
ZET405/488/594x EX ET - 405/488/594nm Laser Triple Band Set for TIRF applications
ZET405/488/594m EM ET - 405/488/594nm Laser Triple Band Set for TIRF applications
ET535/70m EM ET - 405/488/594nm Laser Triple Band Set for TIRF applications
ET450/50m EM ET - 405/488/594nm Laser Triple Band Set for TIRF applications
ET645/75m EM ET - 405/488/594nm Laser Triple Band Set for TIRF applications
ZET442/514/561x EX ET - 442/514/561nm Laser Triple Band Set for TIRF applications
ZT442/514/561rpc BS ET - 442/514/561nm Laser Triple Band Set for TIRF applications
ZET442/514/561m-TRF EM ET - 442/514/561nm Laser Triple Band Set for TIRF applications
ET540/30m EM ET - 442/514/561nm Laser Triple Band Set for TIRF applications
ZT442/514/561rpc BS ET - 442/514/561nm Laser Triple Band Set for TIRF applications
ZET442/514/561m EM ET - 442/514/561nm Laser Triple Band Set for TIRF applications
ZET442/514/561x EX ET - 442/514/561nm Laser Triple Band Set for TIRF applications
ET575lp EM ET - 442/514/561nm Laser Triple Band Set for TIRF applications
ET480/40m EM ET - 442/514/561nm Laser Triple Band Set for TIRF applications
ZT405/488/561/640rpc BS ET - 405/488/561/640nm Laser Quad Band Set for TIRF applications
ZET405/488/561/640x EX ET - 405/488/561/640nm Laser Quad Band Set for TIRF applications
ZET405/488/561/640m-TRF EM ET - 405/488/561/640nm Laser Quad Band Set for TIRF applications
ET450/50m EM ET - 405/488/561/640nm Laser Quad Band Set for TIRF applications
ET525/50m EM ET - 405/488/561/640nm Laser Quad Band Set for TIRF applications
ZET405/488/561/640m EM ET - 405/488/561/640nm Laser Quad Band Set for TIRF applications
ZT405/488/561/640rpc BS ET - 405/488/561/640nm Laser Quad Band Set for TIRF applications
ZET405/488/561/640x EX ET - 405/488/561/640nm Laser Quad Band Set for TIRF applications
ET655lp EM ET - 405/488/561/640nm Laser Quad Band Set for TIRF applications
ET595/50m EM ET - 405/488/561/640nm Laser Quad Band Set for TIRF applications
ZT405/488/561/647rpc BS ET - 405/488/561/647nm Laser Quad Band Set for TIRF applications
ZET405/488/561/647x EX ET - 405/488/561/647nm Laser Quad Band Set for TIRF applications
ZET405/488/561/647m-TRF EM ET - 405/488/561/647nm Laser Quad Band Set for TIRF applications
ET700/50m EM ET - 405/488/561/647nm Laser Quad Band Set for TIRF applications
ZET405/488/561/647m EM ET - 405/488/561/647nm Laser Quad Band Set for TIRF applications
ZET405/488/561/647x EX ET - 405/488/561/647nm Laser Quad Band Set for TIRF applications
ZT405/488/561/647rpc BS ET - 405/488/561/647nm Laser Quad Band Set for TIRF applications
ET450/50m EM ET - 405/488/561/647nm Laser Quad Band Set for TIRF applications
ET525/50m EM ET - 405/488/561/647nm Laser Quad Band Set for TIRF applications
ET605/52m EM ET - 405/488/561/647nm Laser Quad Band Set for TIRF applications